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KMID : 0613820120220050605
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Journal of Life Science
2012 Volume.22 No. 5 p.605 ~ p.612
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Probiotic Potential of Indigenous Bacillus sp. BCNU 9028 Isolated from Meju
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Shin Hwa-Jin
Bang Ji-Hun
Choi Hye-Jung
Kim Dong-Wan
Ahn Cheol-Soo
Jeong Young-Kee
Joo Woo-Hong
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Abstract
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Spore-forming bacteria are being used as probiotic supplements for human and animal use, due to their low pH stability and ability to survive the gastric barrier. In this study, the BCNU 9028 strain was screened from meju, a Korean fermented soybean food starter. Biochemical and physiological characteristics, as well as 16S rDNA sequence analyses, indicate that this strain belongs to the genus Bacillus. Bacillus sp. BCNU 9028 showed a 92% survivability at pH 2.5 and could also withstand 0.3% ox bile. Furthermore, it was postulated that Bacillus sp. BCNU 9028 could prevent biofilm formation and adherence of food-borne pathogens such as Listeria monocytogenes, S. aureus and E. coli on the basis of its autoaggregation and coaggregation capacity with food-borne pathogens. It was shown that BCNU 9028 has good abilities to adhere to the intestinal tract from its hydrophobic character (63.3%). The Bacillus sp. BCNU 9028 strain especially elicited antibacterial activity against both Gram-positive and -negative pathogens. These findings suggested that the Bacillus sp. BCNU 9028 strain could be used as a potential probiotic.
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KEYWORD
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Antibacterial activity, Bacillus sp., coaggregation, Meju, probiotic
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